Protein detail

EHD1

EH domain-containing protein 1 (PAST homolog 1) (hPAST1) (Testilin)

Entry name
EHD1
UniProt ID
EVMP confidence score
0.88
Supporting publications (n)
114
Transmembrane count
Protein classification
Plasma proteinsPredicted intracellular proteinsTransporters
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information11
Protein Names
EH domain-containing protein 1 (PAST homolog 1) (hPAST1) (Testilin)
Protein Class (3)
Plasma proteinsPredicted intracellular proteinsTransporters
Protein Function (2)
  • Transporters:Transporter channels and pores
  • Predicted intracellular proteins
Entrez Gene Symbol
Gene Synonym (5)
FLJ42622FLJ44618H-PASTHPAST1PAST1
Gene Description
EH domain containing 1
Chromosome
11
Position
64851642-64888296
Supporting publications (n)
114
EVMP confidence score
0.88
Fluorescence & Localization2
EHD1 fluorescence
Cell SpecificAstrocytes
Function & Pathway6
Relations & Evidence11

Enzyme-Mediated Modification (1)

1 record.

Substrate Gene SymbolEnzyme Gene SymbolEnzyme UniProt IDResidue TypeResidue OffsetModificationDatabaseReferences
EHD1EGFRP00533Y453phosphorylationKEAKEA:17570479

Ligand-Receptor Signaling (6)

6 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
intracellularintracellularComPPINoNoNoNoNo
intracellularintracellularGO_IntercellNoNoNoNoNo
intracellularintracellularUniProt_locationNoNoNoNoNo
intracellularintracellularOmniPathNoNoNoNoNo
plasma_membraneplasma_membraneUniProt_locationNoNoNoNoNo
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationSize Exclusion ChromatographyMass spectrometryWestern blotting23788664838343172
Sequence, Structure & Domains13

Sequences

Length
534
Mass
60,627
Sequence
MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE

3D Structural Models

Turn
523..525
Helix
440..443; 445..454; 464..473; 478..488; 498..512; 528..530
Beta Strand
458..463; 494..497
3D Structure
NMR spectroscopy (5)

Domain & Motif Annotations

Coiled Coil
198..227
Domain (CC)
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.
Domain (FT)
55..286; Dynamin-type G; 444..532; EH; 476..511; EF-hand
Region
65..72; G1 motif; 91..92; G2 motif; 153..156; G3 motif; 219..222; G4 motif; 243; G5 motif
Protein Families (3)
  • TRAFAC class dynamin-like GTPase superfamily
  • Dynamin/Fzo/YdjA family
  • EHD subfamily
Sequence Similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.
Clinical Relevance4
Interaction Protein (5)
ENSG00000013016ENSG00000099940ENSG00000100139ENSG00000131381ENSG00000185722
Interaction Count
5
Interaction Dataset (2)
intact_biogridintact_biogrid_bioplex
Supporting Publications113
PMIDTitleAbstract
33114768Proteomic Profiling of Two Distinct Populations of Extracellular Vesicles Isolated from Human Seminal Plasma.No abstract available
33580265Proteomic Profile of Urinary Extracellular Vesicles Identifies AGP1 as a Potential Biomarker of Primary Aldosteronism.No abstract available
33709510Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection.No abstract available
33718342Extracellular Vesicles Derived From Adult and Fetal Bone Marrow Mesenchymal Stromal Cells Differentially Promote ex vivo Expansion of Hematopoietic Stem and Progenitor Cells.No abstract available
33729255A new urinary exosome enrichment method by a combination of ultrafiltration and TiO2 nanoparticles.No abstract available
33806028Proteome of Stored RBC Membrane and Vesicles from Heterozygous Beta Thalassemia Donors.No abstract available
33905554Proteomic analysis of extracellular vesicles identified PI3K pathway as a potential therapeutic target for cabazitaxel-resistant prostate cancer.No abstract available
34064456Characterization of Extracellular Vesicle Cargo in Sjögren's Syndrome through a SWATH-MS Proteomics Approach.No abstract available
34071777Proteome Characterization of BALF Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Unveiling Undercover Molecular Pathways.No abstract available
34108659Quantitative proteomics identifies the core proteome of exosomes with syntenin-1 as the highest abundant protein and a putative universal biomarker.Syntenin-1 is also present in exosomes across different species and biofluids, highlighting its potential use as a putative universal biomarker of exosomes. We identified syntenin-1 as a consistently abundant protein in exosomes from different cellular origins.
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