Protein detail

NINJ2

Ninjurin-2 (Nerve injury-induced protein 2)

Entry name NINJ2	UniProt ID Q9NZG7	EVMP confidence score 0.38
Supporting publications (n) 1	Transmembrane count 2	Protein classification

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information7

Protein Names

Ninjurin-2 (Nerve injury-induced protein 2)

Transmembrane

61..92; Helical; Name=Helix alpha3; 97..126; Helical; Name=Helix alpha4

Transmembrane Count

Ensembl

ENSG00000171840

Entrez Gene Symbol

NINJ2

Supporting publications (n)

EVMP confidence score

0.38

Fluorescence & Localization1

Function & Pathway4

Cellular Component

GO:0005886 plasma membrane

Molecular Function

GO:0005515 protein binding

Biological Process (3)

Mediation Categories

Other mediation

Relations & Evidence18

Ligand-Receptor Signaling (16)

16 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
transmembrane	transmembrane	LOCATE	No	No	No	No	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	No	No
transmembrane	transmembrane	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No
transmembrane	transmembrane_predicted	Phobius	No	No	No	No	No

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Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	NINJ2	Q9NZG7	6	PDB	PDB:8szb

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Western blotting	1	40098346

Sequence, Structure & Domains10

Sequences

Length

142

Mass

15,680

Sequence

MESARENIDLQPGSSDPRSQPINLNHYATKKSVAESMLDVALFMSNAMRLKAVLEQGPSSHYYTTLVTLISLSLLLQVVIGVLLVVIARLNLNEVEKQWRLNQLNNAATILVFFTVVINVFITAFGAHKTGFLAARASRNPL

3D Structural Models

Helix

27..36; 40..56; 63..93; 99..125

3D Structure

Electron microscopy (1)

Domain & Motif Annotations

Compositional Bias

12..21; Polar residues

Domain (CC)

Composed of 4 alpha helices: 2 hydrophobic transmembrane regions (alpha3 and alpha4) and 2 alpha helices (alpha1 and alpha2) (PubMed:39667936). Alpha1 and alpha2 feature one hydrophobic side and a hydrophilic side (PubMed:39667936). In contrast to NINJ1, does not disrupt membrane integrity (PubMed:38614101, PubMed:39667936). NINJ2 filaments are curved toward the intracellular space due to cholesterol-binding, preventing circularization and ability to mediate plasma membrane rupture (PubMed:39667936).

Region

1..21; Disordered; 25..37; Helix alpha1; 38..57; Helix alpha2

Protein Families

Ninjurin family

Sequence Similarities

Belongs to the ninjurin family.

Supporting Publications1

PMID	Title	Abstract
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available