Protein detail

CLIC4

Chloride intracellular channel protein 4 (Glutaredoxin-like oxidoreductase CLIC4) (EC 1.8.-.-) (Intracellular chloride ion channel protein p64H1)

Entry name
CLIC4
UniProt ID
Q9Y696
EVMP confidence score
0.88
Supporting publications (n)
41
Transmembrane count
1
Protein classification
Predicted intracellular proteinsTransporters
EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40
Basic Information13
Protein Names
Chloride intracellular channel protein 4 (Glutaredoxin-like oxidoreductase CLIC4) (EC 1.8.-.-) (Intracellular chloride ion channel protein p64H1)
Protein Class (2)
Predicted intracellular proteinsTransporters
Protein Function (2)
  • Transporters:Transporter channels and pores
  • Predicted intracellular proteins
Transmembrane
37..57; Helical; Note=After insertion into the membrane
Transmembrane Count
1
Ensembl
ENSG00000169504
Entrez Gene Symbol
CLIC4
Gene Synonym (5)
CLIC4LDKFZP566G223H1huH1P64H1
Gene Description
Chloride intracellular channel 4
Chromosome
1
Position
24745382-24844321
Supporting publications (n)
41
EVMP confidence score
0.88
Fluorescence & Localization4
CLIC4 fluorescence
Cell SpecificAdrenal medulla cellsBlood Cell SpecificeosinophilBlood Lineage Specificgranulocytes
Function & Pathway6
Protein Function (2)
  • Transporters:Transporter channels and pores
  • Predicted intracellular proteins
Cellular Component (19)
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Molecular Function (3)
Biological Process (3)
Canonical Pathways (3)
  • M5883 Naba secreted factors
  • M5885 Naba matrisome associated
  • M5889 Naba matrisome
Mediation Categories (2)
Clinical-translation mediationFusion and delivery mediation
Relations & Evidence21

Ligand-Receptor Signaling (11)

11 records.

CategoryParentDatabaseTransmitterReceiverSecretedPlasma Membrane (Transmembrane)Plasma Membrane (Peripheral)
plasma_membraneplasma_membraneOmniPathNoNoNoNoNo
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Regulatory Interaction Network (1)

1 record.

Source Protein SymbolSource UniProt IDTarget Protein SymbolTarget UniProt IDIs DirectedIs StimulationIs InhibitionDatabaseReferences
CDK5Q00535CLIC4Q9Y696YesYesNoSIGNORSparser_ProtMapperProtMapperSIGNOR:30237421ProtMapper:30237421

Protein Complex Composition (8)

Isolation & Detection Technology (1)

1 record.

EV Isolation MethodDetection MethodNumber of ReferencesReferences
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion ChromatographyImmunoaffinity CaptureMass spectrometry6381133683732247541068253335925004130796838607062
Sequence, Structure & Domains13

Sequences

Length
253
Mass
28,772
Sequence
MALSMPLNGLKEEDKEPLIELFVKAGSDGESIGNCPFSQRLFMILWLKGVVFSVTTVDLKRKPADLQNLAPGTHPPFITFNSEVKTDVNKIEEFLEEVLCPPKYLKLSPKHPESNTAGMDIFAKFSAYIKNSRPEANEALERGLLKTLQKLDEYLNSPLPDEIDENSMEDIKFSTRKFLDGNEMTLADCNLLPKLHIVKVVAKKYRNFDIPKEMTGIWRYLTNAYSRDEFTNTCPSDKEVEIAYSDVAKRLTK

3D Structural Models

Turn
101..103; 116..120; 244..247
Helix
36..48; 64..69; 88..98; 112..115; 121..130; 134..136; 137..156; 186..206; 215..225; 228..231; 237..243
Beta Strand
19..25; 29..32; 53..57; 77..80; 83..85; 181..183
3D Structure
X-ray crystallography (3)

Domain & Motif Annotations

Motif
35..38; G-site
Domain (CC)
The active G-site contains a monothiol Cys-X-X-Ser motif which mediates glutathione-dependent redox catalysis.; DOMAIN: Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as a chloride channel. The redox status of the active cysteine in Cys-X-X-Cys/Ser motif likely determines the capacity to adopt a soluble or membrane-inserted state. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
Domain (FT)
81..244; GST C-terminal
Region
2..101; Required for insertion into the membrane
Protein Families
Chloride channel CLIC family
Sequence Similarities
Belongs to the chloride channel CLIC family.
Clinical Relevance5
Drug Targets
Literature-reported target
Antibody (2)
HPA008019HPA060804
Interaction Protein
ENSG00000159212
Interaction Count
1
Interaction Dataset
intact_biogrid
Supporting Publications41
PMIDTitleAbstract
30760538Microvesicle Proteomic Profiling of Uterine Liquid Biopsy for Ovarian Cancer Early Detection.No abstract available
31320591Exosomes regulate neurogenesis and circuit assembly.No abstract available
31508500Proteomic profiling of extracellular vesicles allows for human breast cancer subtyping.No abstract available
31588238Dual-platform affinity proteomics identifies links between the recurrence of ovarian carcinoma and proteins released into the tumor microenvironment.No abstract available
32295833Dysregulation of Exosome Cargo by Mutant Tau Expressed in Human-induced Pluripotent Stem Cell (iPSC) Neurons Revealed by Proteomics Analyses.Notably, mTau exosomes (not control exosomes) contain ANP32A (also known as I1PP2A), an endogenous inhibitor of the PP2A phosphatase which regulates the phosphorylation state of p-Tau.
32795414Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.Among traditional exosome markers, CD9, HSPA8, ALIX, and HSP90AB1 represent pan-EVP markers, while ACTB, MSN, and RAP1B are novel pan-EVP markers.
33114768Proteomic Profiling of Two Distinct Populations of Extracellular Vesicles Isolated from Human Seminal Plasma.No abstract available
33709510Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection.No abstract available
33718342Extracellular Vesicles Derived From Adult and Fetal Bone Marrow Mesenchymal Stromal Cells Differentially Promote ex vivo Expansion of Hematopoietic Stem and Progenitor Cells.No abstract available
33729255A new urinary exosome enrichment method by a combination of ultrafiltration and TiO2 nanoparticles.No abstract available
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