Protein detail

EPN1

Epsin-1 (EH domain-binding mitotic phosphoprotein) (EPS-15-interacting protein 1)

Protein symbol EPN1	UniProt ID Q9Y6I3	EVMP score 0.63
Frequency 9	Transmembrane count	Protein classification Plasma proteinsPredicted intracellular proteins

Basic Information

Protein Names

Epsin-1 (EH domain-binding mitotic phosphoprotein) (EPS-15-interacting protein 1)

Protein Class

Plasma proteinsPredicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000063245

Entrez Gene Symbol

EPN1

Gene Description

Epsin 1

Chromosome

Position

55675226-55709858

Frequency

EVMP Score

0.63

Fluorescence & Localization

Tissue Specificheart muscleCell SpecificDecidual stromal cellsSingle-Nuclei Brain Specificoligodendrocyte

Function & Pathway

Protein Function

Predicted intracellular proteins

Cellular Component

Molecular Function

KEGG

hsa04144 Endocytosis

Reactome

Mediation Categories

Fusion and delivery mediationReceptor-signaling mediation

Relations & Evidence

Enzyme-Mediated Modification

3 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
EPN1	CDK1	P06493	S	357	phosphorylation	phosphoELMKEA	KEA:17570479 phosphoELM:10764745 KEA:10764745
EPN1	CDK1	P06493	S	468	phosphorylation	MIMPHPRD_MIMPphosphoELM_MIMPPhosphoSite_MIMP
EPN1	GSK3B	P49841	S	357	phosphorylation	KEA	KEA:17570479

Ligand-Receptor Signaling

11 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

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Regulatory Interaction Network

4 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
EPN1	Q9Y6I3	EGFR	P00533	Yes	No	Yes	WangSIGNORBioGRID	BioGRID:19054389 SIGNOR:19054389
EPN1	Q9Y6I3		COMPLEX:CHEBI:18348_O94973_P09496_P09497_P53675_P53680_P63010_Q00610_Q96CW1	Yes	Yes	No	SIGNOR	SIGNOR:24789820
	COMPLEX:O94973_P53680_P63010_Q96CW1	EPN1	Q9Y6I3	Yes	Yes	No	SIGNOR	SIGNOR:15496985
CDK1	P06493	EPN1	Q9Y6I3	Yes	Yes	Yes	HPRD_MIMPSIGNORProtMapperdbPTMPhosphoSite_KEAphosphoELM_KEAHPRDWangPhosphoSite_ProtMapperNetworKIN_KEAphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetPhosphoPointKEAHPRD_KEAphosphoELMSIGNOR_ProtMapperPhosphoSite	phosphoELM:10764745 HPRD:10764745 PhosphoSite:10764745 KEA:10764745 SIGNOR:10764745 KEA:17570479 ProtMapper:10764745 dbPTM:10764745

Protein Complex Composition

7 records.

Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs
ARHGAP31 CDC42 CDK1 DIAPH2 EPN1 PAK6 PLEKHG2 TBC1D3F	A6NER0 O60879 P06493 P60953 Q2M1Z3 Q9H7P9 Q9NQU5 Q9Y6I3	1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC3724
AP1B1 AP1G1 AP1M1 AP1M2 AP1S1 AP1S2 AP2B1 ASMTL CLINT1 CLTB CLTC EPN1 GGA2 UBC	O43747 O95671 P09497 P0CG48 P56377 P61966 P63010 Q00610 Q10567 Q14677 Q9BXS5 Q9UJY4 Q9Y6I3 Q9Y6Q5	1:1:1:1:1:1:1:1:1:1:1:1:1:1	NetworkBlastCompleat	Compleat:HC4458
EPN1 EPN2 EPN3	O95208 Q9H201 Q9Y6I3	0:0:0	hu.MAP2
CCDC25 EPN1 NDRG2 TYMS	P04818 Q86WR0 Q9UN36 Q9Y6I3	0:0:0:0	hu.MAP2
EPN1 NDRG2 TYMS	P04818 Q9UN36 Q9Y6I3	0:0:0	hu.MAP2
EPN1 UROD	P06132 Q9Y6I3	0:0	hu.MAP
CCNB1 EPN1	P14635 Q9Y6I3	0:0	hu.MAP2

Isolation & Detection Technology

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion Chromatography	Mass spectrometry [LTQ-FT Ultra]Mass spectrometry	11	36064647 40189497 38895962 41068253 33592500 30915084 33309826 37670049 37223008 36982312 37438638

Sequence, Structure & Domains

Sequences

Length

576

Mass

60,293

Sequence

MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQPPSCGPEDDAQLQLALSLSREEHDKEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTAPAPAPTTDPWGGPAPMAAAVPTAAPTSDPWGGPPVPPAADPWGGPAPTPASGDPWRPAAPAGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAFSDPWGGSPAKPSTNGTTAAGGFDTEPDEFSDFDRLRTALPTSGSSAGELELLAGEVPARSPGAFDMSGVRGSLAEAVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGAKASNPFLPGGGPATGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL

Alternative Products

Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=Q9Y6I3-2; Sequence=Displayed; Name=2; IsoId=Q9Y6I3-1; Sequence=VSP_041010, VSP_041011; Name=3; IsoId=Q9Y6I3-3; Sequence=VSP_041011, VSP_041012

Alternative Sequence

1; M -> MGDQSWLWNQAAPGVRSPVFACSVEKGNVPLVLSEHLAHSRDPGSGAVRFLISPEPWASAILGTSGLLASPVLPAALDAVTCQHLPQPSSGSRPISPRIGALCPLLLQPGTM (in isoform 2); 202..226; Missing (in isoform 2 and isoform 3); 393; Missing (in isoform 3)

3D Structural Models

Helix

20..27; 39..47; 51..62; 63..65; 72..86; 90..98; 100..108; 121..134

3D Structure

NMR spectroscopy (1); X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias

157..167; Low complexity; 265..296; Low complexity; 297..314; Pro residues; 332..368; Low complexity; 454..468; Pro residues; 557..570; Pro residues

Repeat

274..276; 1; 294..296; 2; 306..308; 3; 319..321; 4; 332..334; 5; 349..351; 6; 367..369; 7; 377..379; 8; 502..504; 1; 518..520; 2; 572..574; 3

Motif

402..411; [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif

Domain (CC)

The NPF repeat domain is involved in EPS15 binding.; DOMAIN: The DPW repeat domain is involved in AP2A2 and clathrin binding.; DOMAIN: The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1..

Domain (FT)

12..144; ENTH; 183..202; UIM 1; 208..227; UIM 2; 233..252; UIM 3

Region

149..185; Disordered; 265..404; Disordered; 274..379; 8 X 3 AA repeats of [ED]-P-W; 448..576; Disordered; 502..574; 3 X 3 AA repeats of N-P-F

Protein Families

Epsin family

Sequence Similarities

Belongs to the epsin family.

Clinical Relevance

Antibody

CAB009729 HPA061136 HPA071503

Interaction Protein

ENSG00000085832ENSG00000092621ENSG00000169891

Interaction Count

Interaction Dataset

intact_biogridintact_biogrid_opencell