EV MemProt Atlas

Basic Information

Protein Names

Actin-binding protein WASF2 (Protein WAVE-2) (Verprolin homology domain-containing protein 2) (Wiskott-Aldrich syndrome protein family member 2) (WASP family protein member 2)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000158195

Entrez Gene Symbol

WASF2

Gene Synonym (2)

SCAR2WAVE2

Gene Description

WASP family member 2

Chromosome

1

Position

27404230-27490167

Supporting publications (n)

24

EVMP confidence score

0.63

Fluorescence & Localization

Function & Pathway

Protein Function

Predicted intracellular proteins

Cellular Component (11)

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Molecular Function (7)

Biological Process (3)

KEGG (8)

Reactome (14)

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Mediation Categories (4)

Clinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence27

Enzyme-Mediated Modification (11)

11 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
WASF2	ABL1	P00519	Y	150	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPProtMapperRLIMS-P_ProtMapperReactome_ProtMapperKEAphosphoELMREACH_ProtMapper	ProtMapper:21419341 ProtMapper:20533885 ProtMapper:16899465 ProtMapper:23354023 KEA:15657136 phosphoELM:15657136 ProtMapper:22448228
WASF2	CSNK2A1	P68400	S	482	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317 SIGNOR:19012317
WASF2	CSNK2A1	P68400	S	484	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317 SIGNOR:19012317
WASF2	CSNK2A1	P68400	S	488	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317 SIGNOR:19012317
WASF2	CSNK2A1	P68400	S	497	phosphorylation	PhosphoSite_MIMPMIMPProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317
WASF2	CSNK2A1	P68400	S	489	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
WASF2	MAPK3	P27361	S	343	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
WASF2	MAPK3	P27361	S	351	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
WASF2	MAPK3	P27361	T	346	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
WASF2	MAPK1	P28482	S	308	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper

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Ligand-Receptor Signaling (8)

8 records.

Category	Parent	Database
intracellular	intracellular	LOCATE
intracellular	intracellular	ComPPI
intracellular	intracellular	GO_Intercell
intracellular	intracellular	UniProt_location
intracellular	intracellular	OmniPath
plasma_membrane	plasma_membrane	UniProt_location
basolateral_cell_membrane	plasma_membrane	UniProt_location
plasma_membrane	plasma_membrane	OmniPath

Regulatory Interaction Network (5)

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
ABL1	P00519	WASF2	Q9Y6W5	Yes	Yes		WangSparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperHPRDHINTRLIMS-P_ProtMapperKEAReactome_ProtMapperphosphoELM_KEAphosphoELMSPIKE_LCREACH_ProtMapperSPIKE	ProtMapper:21419341 HINT:35271311 ProtMapper:34022881 KEA:15657136 ProtMapper:20533885 SPIKE_LC:20841568 phosphoELM:15657136 ProtMapper:33869225 HPRD:17101133 ProtMapper:16899465 SPIKE:20841568 SIGNOR:16899465 ProtMapper:23354023 HINT:37219487 SPIKE_LC:15657136 ProtMapper:22448228 SPIKE:15657136
BAIP2	Q9UQB8	WASF2	Q9Y6W5	Yes	Yes		DOMINOWangSIGNORHPRDHINTACSNSPIKE_LCLit-BM-17	SIGNOR:11130076 ACSN:12054568 HPRD:12054568 Lit-BM-17:26186194 HINT:35271311 ACSN:16702231 Lit-BM-17:11130076 DOMINO:11130076 Lit-BM-17:26496610 SPIKE_LC:11130076 HINT:11130076 SPIKE_LC:16713569 HINT:33961781 HPRD:11130076 HINT:26496610 ACSN:9843499
CSK21	P68400	WASF2	Q9Y6W5	Yes		Yes	PhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317 PhosphoSite:19012317 SIGNOR:19012317
DTBP1	Q96EV8	WASF2	Q9Y6W5	Yes	Yes		SIGNOR	SIGNOR:20531346
MK03	P27361	WASF2	Q9Y6W5	Yes			PhosphoSite_MIMPMIMPPhosphoSite_norefiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:24415754 PhosphoSite:25342748

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
ABI3-WAVE2-CYFIP1 complex	ABI3 CYFIP1 WASF2	Q7L576 Q9P2A4 Q9Y6W5	0:0:0	CORUM	CORUM:6991	28978070
	PRKACA SORBS3 WASF2	O60504 P17612 Q9Y6W5	1:1:1	CompleatCFinder	Compleat:HC5921

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometry	2	38113368 38207106

Sequence, Structure & Domains

Sequences

Length

498

Mass

54,284

Sequence

MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9Y6W5-1; Sequence=Displayed; Name=2; IsoId=Q9Y6W5-2; Sequence=VSP_042514, VSP_042515

Alternative Sequence

275..281; SYPVDNQ -> RFSAAQG (in isoform 2); 282..498; Missing (in isoform 2)

3D Structural Models

Helix

436..446

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias

252..263; Low complexity; 298..407; Pro residues

Domain (CC)

Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain.

Domain (FT)

436..453; WH2

Region

173..203; Disordered; 240..435; Disordered

Protein Families

SCAR/WAVE family

Sequence Similarities

Belongs to the SCAR/WAVE family.

Clinical Relevance

Antibody (2)

CAB022632 HPA045288

Interaction Protein (16)

ENSG00000055163ENSG00000061676ENSG00000075624ENSG00000097007ENSG00000108518ENSG00000108798ENSG00000112290ENSG00000120860ENSG00000136754ENSG00000138443ENSG00000162522ENSG00000166913ENSG00000175866ENSG00000186908ENSG00000204131ENSG00000254999

Interaction Count

16

Interaction Dataset (4)

intact_biogrid_opencellbiogrid_opencellintact_biogridbiogrid_opencell_bioplex

Supporting Publications24

PMID	Title	Related sentences
31805958	Proteomic analysis of cerebrospinal fluid extracellular vesicles reveals synaptic injury, inflammation, and stress response markers in HIV patients with cognitive impairment.	No related sentences available
32560723	T2 and T17 cytokines alter the cargo and function of airway epithelium-derived extracellular vesicles.	No related sentences available
32916986	Proteomic Approach for Searching for Universal, Tissue-Specific, and Line-Specific Markers of Extracellular Vesicles in Lung and Colorectal Adenocarcinoma Cell Lines.	No related sentences available
33304478	The proteomic landscape of small urinary extracellular vesicles during kidney transplantation.	No related sentences available
33592500	A Proteomic Approach to Understand the Clinical Significance of Acute Myeloid Leukemia-Derived Extracellular Vesicles Reflecting Essential Characteristics of Leukemia.	No related sentences available
33936570	Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer-based precipitation and size exclusion chromatography.	No related sentences available
34473505	Column-based Technology for CD9-HPLC Immunoaffinity Isolation of Serum Extracellular Vesicles.	Column-based Technology for CD9-HPLC Immunoaffinity Isolation of Serum Extracellular Vesicles.
34576246	Burn Injury Induces Proinflammatory Plasma Extracellular Vesicles That Associate with Length of Hospital Stay in Women: CRP and SAA1 as Potential Prognostic Indicators.	No related sentences available
36064647	Systemic proteomics and miRNA profile analysis of exosomes derived from human pluripotent stem cells.	No related sentences available
36146834	Human Cytomegalovirus Modifies Placental Small Extracellular Vesicle Composition to Enhance Infection of Fetal Neural Cells In Vitro.	No related sentences available

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Entry name WASF2	UniProt ID Q9Y6W5	EVMP confidence score 0.63
Supporting publications (n) 24	Transmembrane count	Protein classification Predicted intracellular proteins