Protein detail

WASF2

Actin-binding protein WASF2 (Protein WAVE-2) (Verprolin homology domain-containing protein 2) (Wiskott-Aldrich syndrome protein family member 2) (WASP family protein member 2)

Entry name WASF2	UniProt ID Q9Y6W5	EVMP confidence score 0.88
Supporting publications (n) 24	Transmembrane count	Protein classification Predicted intracellular proteins

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information11

Protein Names

Actin-binding protein WASF2 (Protein WAVE-2) (Verprolin homology domain-containing protein 2) (Wiskott-Aldrich syndrome protein family member 2) (WASP family protein member 2)

Protein Class

Predicted intracellular proteins

Protein Function

Predicted intracellular proteins

Ensembl

ENSG00000158195

Entrez Gene Symbol

WASF2

Gene Synonym (2)

SCAR2WAVE2

Gene Description

WASP family member 2

Chromosome

Position

27404230-27490167

Supporting publications (n)

EVMP confidence score

0.88

Fluorescence & Localization1

Function & Pathway7

Protein Function

Predicted intracellular proteins

Cellular Component (11)

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Molecular Function (7)

Biological Process (3)

KEGG (8)

Reactome (14)

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Mediation Categories (4)

Clinical-translation mediationFusion and delivery mediationImmune mediationReceptor-signaling mediation

Relations & Evidence27

Enzyme-Mediated Modification (11)

11 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
WASF2	ABL1	P00519	Y	150	phosphorylation	Sparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPProtMapperRLIMS-P_ProtMapperReactome_ProtMapperKEAphosphoELMREACH_ProtMapper	ProtMapper:21419341 ProtMapper:20533885 ProtMapper:16899465 ProtMapper:23354023 KEA:15657136 phosphoELM:15657136 ProtMapper:22448228
WASF2	CSNK2A1	P68400	S	482	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317 SIGNOR:19012317
WASF2	CSNK2A1	P68400	S	484	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317 SIGNOR:19012317
WASF2	CSNK2A1	P68400	S	488	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPSIGNORProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317 SIGNOR:19012317
WASF2	CSNK2A1	P68400	S	497	phosphorylation	PhosphoSite_MIMPMIMPProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317
WASF2	CSNK2A1	P68400	S	489	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
WASF2	MAPK3	P27361	S	343	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
WASF2	MAPK3	P27361	S	351	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
WASF2	MAPK3	P27361	T	346	phosphorylation	PhosphoSite_MIMPMIMPProtMapperPhosphoSitePhosphoSite_ProtMapper
WASF2	MAPK1	P28482	S	308	phosphorylation	PhosphoSite_MIMPMIMPHPRD_MIMPProtMapperPhosphoSitePhosphoSite_ProtMapper

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Ligand-Receptor Signaling (8)

8 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
intracellular	intracellular	LOCATE	No	No	No	No	No
intracellular	intracellular	ComPPI	No	No	No	No	No
intracellular	intracellular	GO_Intercell	No	No	No	No	No
intracellular	intracellular	UniProt_location	No	No	No	No	No
intracellular	intracellular	OmniPath	No	No	No	No	No
plasma_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
basolateral_cell_membrane	plasma_membrane	UniProt_location	No	No	No	No	No
plasma_membrane	plasma_membrane	OmniPath	No	No	No	No	No

Regulatory Interaction Network (5)

5 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
ABL1	P00519	WASF2	Q9Y6W5	Yes	Yes	No	WangSparser_ProtMapperphosphoELM_MIMPPhosphoSite_MIMPMIMPiPTMnetSIGNORProtMapperHPRDHINTRLIMS-P_ProtMapperKEAReactome_ProtMapperphosphoELM_KEAphosphoELMSPIKE_LCREACH_ProtMapperSPIKE	ProtMapper:21419341 HINT:35271311 ProtMapper:34022881 KEA:15657136 ProtMapper:20533885 SPIKE_LC:20841568 phosphoELM:15657136 ProtMapper:33869225 HPRD:17101133 ProtMapper:16899465 SPIKE:20841568 SIGNOR:16899465 ProtMapper:23354023 HINT:37219487 SPIKE_LC:15657136 ProtMapper:22448228 SPIKE:15657136
BAIP2	Q9UQB8	WASF2	Q9Y6W5	Yes	Yes	No	DOMINOWangSIGNORHPRDHINTACSNSPIKE_LCLit-BM-17	SIGNOR:11130076 ACSN:12054568 HPRD:12054568 Lit-BM-17:26186194 HINT:35271311 ACSN:16702231 Lit-BM-17:11130076 DOMINO:11130076 Lit-BM-17:26496610 SPIKE_LC:11130076 HINT:11130076 SPIKE_LC:16713569 HINT:33961781 HPRD:11130076 HINT:26496610 ACSN:9843499
CSK21	P68400	WASF2	Q9Y6W5	Yes	No	Yes	PhosphoSite_MIMPMIMPHPRD_MIMPPhosphoSite_norefSIGNORiPTMnetProtMapperSIGNOR_ProtMapperPhosphoSitePhosphoSite_ProtMapper	ProtMapper:19012317 PhosphoSite:19012317 SIGNOR:19012317
DTBP1	Q96EV8	WASF2	Q9Y6W5	Yes	Yes	No	SIGNOR	SIGNOR:20531346
MK03	P27361	WASF2	Q9Y6W5	Yes	No	No	PhosphoSite_MIMPMIMPPhosphoSite_norefiPTMnetProtMapperPhosphoSitePhosphoSite_ProtMapper	PhosphoSite:24415754 PhosphoSite:25342748

Protein Complex Composition (2)

2 records.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
ABI3-WAVE2-CYFIP1 complex	ABI3 CYFIP1 WASF2	Q7L576 Q9P2A4 Q9Y6W5	0:0:0	CORUM	CORUM:6991	28978070
	PRKACA SORBS3 WASF2	O60504 P17612 Q9Y6W5	1:1:1	CompleatCFinder	Compleat:HC5921

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationSize Exclusion ChromatographyImmunoaffinity Capture	Mass spectrometry	2	38113368 38207106

Sequence, Structure & Domains13

Sequences

Length

498

Mass

54,284

Sequence

MPLVTRNIEPRHLCRQTLPSVRSELECVTNITLANVIRQLGSLSKYAEDIFGELFTQANTFASRVSSLAERVDRLQVKVTQLDPKEEEVSLQGINTRKAFRSSTIQDQKLFDRNSLPVPVLETYNTCDTPPPLNNLTPYRDDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEKLGTSGYPPTLVYQNGSIGCVENVDASSYPPPPQSDSASSPSPSFSEDNLPPPPAEFSYPVDNQRGSGLAGPKRSSVVSPSHPPPAPPLGSPPGPKPGFAPPPAPPPPPPPMIGIPPPPPPVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPTLPPPPLSQPTGGAPPPPPPPPPPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9Y6W5-1; Sequence=Displayed; Name=2; IsoId=Q9Y6W5-2; Sequence=VSP_042514, VSP_042515

Alternative Sequence

275..281; SYPVDNQ -> RFSAAQG (in isoform 2); 282..498; Missing (in isoform 2)

3D Structural Models

Helix

436..446

3D Structure

X-ray crystallography (1)

Domain & Motif Annotations

Compositional Bias

252..263; Low complexity; 298..407; Pro residues

Domain (CC)

Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain.

Domain (FT)

436..453; WH2

Region

173..203; Disordered; 240..435; Disordered

Protein Families

SCAR/WAVE family

Sequence Similarities

Belongs to the SCAR/WAVE family.

Clinical Relevance4

Antibody (2)

CAB022632 HPA045288

Interaction Protein (16)

ENSG00000055163ENSG00000061676ENSG00000075624ENSG00000097007ENSG00000108518ENSG00000108798ENSG00000112290ENSG00000120860ENSG00000136754ENSG00000138443ENSG00000162522ENSG00000166913ENSG00000175866ENSG00000186908ENSG00000204131ENSG00000254999

Interaction Count

Interaction Dataset (4)

intact_biogrid_opencellbiogrid_opencellintact_biogridbiogrid_opencell_bioplex

Supporting Publications24

PMID	Title	Abstract
37204515	Proteomic profiling and functional characterization of serum-derived extracellular vesicles in the mucinous and non-mucinous colon adenocarcinoma.	No abstract available
37322475	Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration.	No abstract available
37926756	Extracellular vesicles from non-neuroendocrine SCLC cells promote adhesion and survival of neuroendocrine SCLC cells.	No abstract available
38014595	Proteome and immune responses of extracellular vesicles derived from macrophages infected with the periodontal pathogen Tannerella forsythia.	No abstract available
38113368	In-Depth Proteome Profiling of Small Extracellular Vesicles Isolated from Cancer Cell Lines and Patient Serum.	No abstract available
38207106	Proteomic, Metabolomic, and Fatty Acid Profiling of Small Extracellular Vesicles from Glioblastoma Stem-Like Cells and Their Role in Tumor Heterogeneity.	No abstract available
38895962	A fast and sensitive size-exclusion chromatography method for plasma extracellular vesicle proteomic analysis.	No abstract available
39207047	The trajectory of vesicular proteomic signatures from HBV-HCC by chitosan-magnetic bead-based separation and DIA-proteomic analysis.	No abstract available
40189497	Small extracellular vesicle-based one-step high-throughput microfluidic platform for epithelial ovarian cancer diagnosis.	No abstract available
40465195	Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.	No abstract available

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