Protein detail

TIGIT

T-cell immunoreceptor with Ig and ITIM domains (V-set and immunoglobulin domain-containing protein 9) (V-set and transmembrane domain-containing protein 3)

Entry name TIGIT	UniProt ID Q495A1	EVMP confidence score 0.25
Supporting publications (n) 1	Transmembrane count 1	Protein classification

EVMP confidence score

Annotation confidence score; open for threshold definitions.

Extremely high >= 0.85High >= 0.70Medium >= 0.55Low >= 0.40

Basic Information7

Protein Names

T-cell immunoreceptor with Ig and ITIM domains (V-set and immunoglobulin domain-containing protein 9) (V-set and transmembrane domain-containing protein 3)

Transmembrane

142..162; Helical

Transmembrane Count

Ensembl

ENSG00000181847

Entrez Gene Symbol

TIGIT

Supporting publications (n)

EVMP confidence score

0.25

Fluorescence & Localization5

Tissue SpecificlungCell SpecificAlveolar cells type 1Single-Nuclei Brain Specificendothelial cellBlood Cell SpecificbasophilBlood Lineage Specificgranulocytes

Function & Pathway5

Cellular Component (2)

Molecular Function (3)

Biological Process (3)

KEGG (2)

Mediation Categories (3)

Adhesion and uptake mediationClinical-translation mediationImmune mediation

Relations & Evidence43

Enzyme-Mediated Modification (2)

2 records.

Substrate Gene Symbol	Enzyme Gene Symbol	Enzyme UniProt ID	Residue Type	Residue Offset	Modification	Database	References
TIGIT	LCK	P06239	Y	225	phosphorylation	PhosphoSite
TIGIT	FYN	P06241	Y	225	phosphorylation	PhosphoSite

Ligand-Receptor Signaling (36)

36 records.

Category	Parent	Database	Transmitter	Receiver	Secreted	Plasma Membrane (Transmembrane)	Plasma Membrane (Peripheral)
cell_adhesion	cell_adhesion	Cellinker	Yes	Yes	No	Yes	No
adhesion	adhesion	OmniPath	Yes	Yes	No	Yes	No
cell_adhesion	cell_adhesion	OmniPath	Yes	Yes	No	Yes	No
transmembrane	transmembrane	UniProt_location	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_topology	No	No	No	Yes	No
transmembrane	transmembrane	UniProt_keyword	No	No	No	Yes	No
transmembrane_predicted	transmembrane	OmniPath	No	No	No	Yes	No
transmembrane	transmembrane	CellPhoneDB	No	No	No	Yes	No
transmembrane	transmembrane	TopDB	No	No	No	Yes	No
transmembrane	transmembrane	Ramilowski_location	No	No	No	Yes	No

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Regulatory Interaction Network (3)

3 records.

Source Protein Symbol	Source UniProt ID	Target Protein Symbol	Target UniProt ID	Is Directed	Is Stimulation	Is Inhibition	Database	References
TIGIT	Q495A1	ARRB2	P32121	Yes	Yes	No	InnateDBSIGNOR	InnateDB:24817116 SIGNOR:24817116
PVR	P15151	TIGIT	Q495A1	Yes	Yes	No	CellChatDBCellPhoneDBUniProt_LRdbICELLNETSIGNORconnectomeDB2020HINTWojtowicz2020IntActLit-BM-17LRdb	connectomeDB2020:19815499 IntAct:21982860 HINT:21982860 ICELLNET:27192565 HINT:28515320 HINT:22421438 IntAct:28515320 Lit-BM-17:21982860 SIGNOR:30591568
LCK	P06239	TIGIT	Q495A1	Yes	No	No	PhosphoSitePhosphoSite_ProtMapperProtMapper	PhosphoSite:23154388

Protein Complex Composition (1)

1 record.

Component Name	Component Gene Symbols	Component UniProt ID	Stoichiometry	Database	Database IDs	References
	TIGIT	Q495A1	2	PDB	PDB:8jenPDB:3rq3PDB:7vytPDB:8jeoPDB:8vtePDB:8szyPDB:3q0hPDB:3ucrPDB:8jel

Isolation & Detection Technology (1)

1 record.

EV Isolation Method	Detection Method	Number of References	References
Differential UltracentrifugationUltrafiltration / Tangential Flow FiltrationSize Exclusion Chromatography	Mass spectrometry [LTQ-FT Ultra]	12	39290459 34071777 25471207 26801919 30353492 36362114 38490958 39104279 30760538 37922300 40091455 38612515

Sequence, Structure & Domains13

Sequences

Length

244

Mass

26,319

Sequence

MRWCLLLIWAQGLRQAPLASGMMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIPLLGAMAATLVVICTAVIVVVALTRKKKALRIHSVEGDLRRKSAGQEEWSPSAPSPPGSCVQAEAAPAGLCGEQRGEDCAELHDYFNVLSYRSLGNCSFFTETG

Alternative Products

Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q495A1-1; Sequence=Displayed; Name=2; IsoId=Q495A1-2; Sequence=VSP_027634, VSP_027635

Alternative Sequence

167..170; KKAL -> FVCF (in isoform 2); 171..244; Missing (in isoform 2)

3D Structural Models

Turn

71..73; 82..84

Helix

79..81; 100..102

Beta Strand

26..28; 31..36; 41..43; 46..49; 53..61; 64..70; 74..77; 85..87; 93..95; 104..113; 116..127

3D Structure

X-ray crystallography (11)

Domain & Motif Annotations

Motif

229..234; ITIM motif

Domain (CC)

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Domain (FT)

22..124; Ig-like V-type

Region

32..42; Homodimerization

Clinical Relevance3

Related Diseases (4)

Cervical cancer Lung cancer Non-small-cell lung cancer Solid tumour/cancer

Biomarker

Phase 2; Phase 3

Drugs (8)

VIBOSTOLIMAB ETIGILIMAB IBI-939 DOMVANALIMAB ANTI-TIGIT MONOCLONAL ANTIBODY ASP8374 ASP-8374 OCIPERLIMAB TIRAGOLUMAB

Supporting Publications1

PMID	Title	Abstract
27863537	High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources.	No abstract available